Profiyanti H. Suharti, Sri Rulianah, Yanty Maryanty, Bambang Irawan, Frederick Chen, Ming-Jinn Tsai
Phanerochaete chrysosporium (P. chrysosporium) has been used in fermentation processes for the production of patchouli oil. The principle of fermentation in isolation patchouli oil is by breaking the cell walls of patchouli leaves using enzymes that found in the P. chrysosporium. Those enzymes have capability to degrading lignin and cellulose in the fermentation process to get better patchouli oil rendemen. The purpose of this study was to determine the effect of fermentation time and the amount of inducer used in the fermentation process to the degradation of cellulose. Fermentation for the production of patchouli oil was done by using the media NLM (Nitrogen Limited Media) and added carboxymethyl cellulose (CMC) as inducer. Cellulose content in the patchouli leaves and fermented product was analyzed by Van Soest method. Cellulose content decreased with the addition of fermentation time and the addition of CMC was increased. The addition of 0.8% w/w CMC cause the highest cellulose degradation (75,26% degradation) with a fermentation time of 21 days. © 2017 American Scientific Publishers All rights reserved.
Department of Chemical Engineering, State Polytechnic of Malang, Malang, 65141, Indonesia; Department of Mechanical Engineering, State Polytechnic of Malang, Malang, 65141, Indonesia
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