Identification of alkaloid compounds in papaya leaves extract (Carica papaya L.) with LC-MS/MS

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Yenny Puspitasari, Yuly Peristiowati, Kaliawan

2017 Indian Journal of Agricultural Biochemistry Vol. 30 Issue 2 Article Cited by 2 Quartile

Abstract

Papaya (Carica papaya L.) contains alkaloid compounds, saponins and flavonoids. The purpose of this study was to identify the alkaloid compounds in papaya leaves extract using LC-MS/MS analysis. The carpaine identification was using Hypersild Gold column with 0.1% formic acid mixture in water and 0.1% formic acid in acetonitrile with 0.3 ml/min flow rate. ESI was used in positive ion mode with SRM (Selected Reaction Monitoring) method. The target carpaine compounds were observed at 479 m/z as precursor ions and at 240 m/z as product ions. © 2017, Indian Society of Agricultural Biochemists. All rights reserved.

Affiliations

Surya Mitra Husada Health School, Kediri, Indonesia; Department of Chemistry, Malang State Polytechnic, Malang, Indonesia