Yanty Maryanty, Tri Adryati, Suharjono, Sutiman Bambang Sumitro
This study aims to predict the degradation mechanism of cellulose substrate in silico from the results of isolation and test the potential of cellulolytic bacteria from rice fields in Greater Malang in producing cellulolytic enzymes, among others: β-1, 4 exoglunase, β-1, 4 endoglucase and β - glucosidase, identified isolates that have high potential in producing cellulolytic enzymes based on 16S rDNA ie isolates A, B, D and F. Only Isolate B was successfully predicted by its substrate enzyme interaction both homologically and based on the results of the isolation of the cellulite gene. Homology B isolate analysis results showed that the hydrogen bonds that occur in glutamic acid GLU257, tryptophan 207, serine 264 and glutamic acid 169, while hydrophobic interactions occur in tryptophan 207 bonds. While the results of the analysis based on isolation of cellulase encoding genes from isolate B were predicted in-silico showed the interaction between hydrogen bonds in tyrosin (TRY 299), glutamine (GLU201 and GLU 342.72), Asparagine (ASN 200) and glycine (GLY 384). This interaction is slightly different from the insilico results obtained homologically i.e there is no interaction with serine and glutamate acid, but with asparagine. Isolate B predicted that its homology in silico can degrade cellulose substrate with the binding affinity of -7.0 Kcal/mol while based on the results of isolation of cellulase encoding genes shows the degradation ability of cellulose substrate with the binding affinity of -6.5 Kcal/mol. © Published under licence by IOP Publishing Ltd.
Department of Industrial Chemical Industry, State Polytechnic of Malang, Jl. Soekarno Hatta No.9, Jatimulyo, Kec. Lowokwaru, Kota Malang, Jawa Timur, 65141, Indonesia